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Notes:
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Producer, John Morrey. The necessity to subclone fragments into appropriate vectors designed for sequencing can be substituted with the use of PCR. In the video, the plus and minus strands of PCR-amplified DNA were separated from each other. Magnetic beads coated with streptavidin were used to capture one of the PCR strands which had been labeled with biotin during the PCR. The strands were separated from each other by washing one strand away from the other strand which was attached to the magnetic beads. These strands served a
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